Name | Mr. Sayan Kundu |
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Organization | University Of Florida |
Position | Graduate Student |
Invited | Yes |
Type | Oral |
Topic | Biochemistry / Chem Bio. |
Title | Labeling cell surface glycosylphosphatidylinositol-anchored proteins through metabolic engineering using an azide-modified phosphatidylinositol |
Author(s) | Sayan Kundu,† Mohit Jaiswal,† Kendall C. Craig, Jiatong Guo, and Zhongwu Guo* |
Author Location(s) | University of Florida |
Abstract | Glycosylphosphatidylinositol (GPI) anchorage is one of the most common mechanisms to attach proteins to the plasma membrane of eukaryotic cells. GPI-anchored proteins (GPI-APs) play a critical role in many biological processes but are difficult to study. Here, a new method was developed for the effective and selective metabolic engineering and labeling of cell surface GPI-APs with an azide-modified phosphatidylinositol (PI) as the biosynthetic precursor of GPIs. It was demonstrated that this azido-PI derivative was taken up by HeLa cells and incorporated into the biosynthetic pathway of GPIs to present azide-labeled GPI-APs on the live cell surface. The azido group was used as a molecular handle to install other labels through a biocompatible click reaction to enable various biological studies, e.g., fluorescent imaging and protein pull-down, which can help explore the functions of GPI-APs and discover new GPI-APs. |
Comments | In this project, we have developed a novel method for selective labeling, enrichment, and |
Date | 06/02/2023 |
Time | 02:15 PM |